蛋白酶 K 来源于林伯氏白色念球菌
Proteinase K from Tritirachium album
| CAS NO： | 39450-01-6 |
| 规格： | ，层析纯,30u/mg，试剂级,600u/ml |
| 包装规格： | 15 ML，250 ML，5 ML，1 G，10 MG，100 MG，25 MG，5 MG，500 MG，250 MG，5 G，1 ML，100毫克，1克，500毫克，1毫升，2毫升，5毫升，250MG，500MG，50MG，25MG，100MG，500 MG，1G |
| 优惠价： | QQ在线咨询在线留言 |
产品价格
| 产品编号 | 品牌 | 纯度规格 | 包装单位 | 单价(元) | 现货 |
| 3208788 | Sigma-Aldrich | Reagents designed and manufactured under current ISO 13485 certification. | 15 ML | 10730 | 请咨询 |
| 3208788 | Sigma-Aldrich | Reagents designed and manufactured under current ISO 13485 certification. | 250 ML | 34260 | 请咨询 |
| 3208788 | Sigma-Aldrich | Reagents designed and manufactured under current ISO 13485 certification. | 5 ML | 4050 | 请咨询 |
| 3208789 | Sigma-Aldrich | Reagents designed and manufactured under current ISO 13485 certification. | 1 G | 19180 | 请咨询 |
| 3208789 | Sigma-Aldrich | Reagents designed and manufactured under current ISO 13485 certification. | 10 MG | 1096 | 请咨询 |
| 3208789 | Sigma-Aldrich | Reagents designed and manufactured under current ISO 13485 certification. | 100 MG | 2550 | 请咨询 |
| 3208789 | Sigma-Aldrich | Reagents designed and manufactured under current ISO 13485 certification. | 25 MG | 1290 | 请咨询 |
| 3208789 | Sigma-Aldrich | Reagents designed and manufactured under current ISO 13485 certification. | 5 MG | 895 | 请咨询 |
| 3208789 | Sigma-Aldrich | Reagents designed and manufactured under current ISO 13485 certification. | 500 MG | 10370 | 请咨询 |
| 3208790 | Sigma-Aldrich | Reagents designed and manufactured under current ISO 13485 certification. | 1 G | 15060 | 请咨询 |
| 3208790 | Sigma-Aldrich | Reagents designed and manufactured under current ISO 13485 certification. | 10 MG | 889 | 请咨询 |
| 3208790 | Sigma-Aldrich | Reagents designed and manufactured under current ISO 13485 certification. | 100 MG | 2210 | 请咨询 |
| 3208790 | Sigma-Aldrich | Reagents designed and manufactured under current ISO 13485 certification. | 25 MG | 1102 | 请咨询 |
| 3208790 | Sigma-Aldrich | Reagents designed and manufactured under current ISO 13485 certification. | 5 MG | 780 | 请咨询 |
| 3208790 | Sigma-Aldrich | Reagents designed and manufactured under current ISO 13485 certification. | 500 MG | 8760 | 请咨询 |
| 3208791 | Sigma-Aldrich | Reagents designed and manufactured under current ISO 13485 certification. | 1 G | 4450 | 请咨询 |
| 3208791 | Sigma-Aldrich | Reagents designed and manufactured under current ISO 13485 certification. | 250 MG | 1610 | 请咨询 |
| 3208791 | Sigma-Aldrich | Reagents designed and manufactured under current ISO 13485 certification. | 5 G | 17730 | 请咨询 |
| 3208792 | Sigma-Aldrich | Reagents designed and manufactured under current ISO 13485 certification. | 1 ML | 1320 | 请咨询 |
| 3208792 | Sigma-Aldrich | Reagents designed and manufactured under current ISO 13485 certification. | 5 ML | 3700 | 请咨询 |
| 3208793 | Sigma-Aldrich | Reagents designed and manufactured under current ISO 13485 certification. | 1 ML | 1350 | 请咨询 |
| 3208794 | Reagents designed and manufactured under current ISO 13485 certification. | 100 MG | 491 | 请咨询 | |
| 3208795 | Sigma-Aldrich | Reagents designed and manufactured under current ISO 13485 certification. | 1 G | 11210 | 请咨询 |
| 3208795 | Sigma-Aldrich | Reagents designed and manufactured under current ISO 13485 certification. | 100 MG | 2000 | 请咨询 |
| 3208795 | Sigma-Aldrich | Reagents designed and manufactured under current ISO 13485 certification. | 25 MG | 922 | 请咨询 |
| 3208796 | Sigma-Aldrich | Reagents designed and manufactured under current ISO 13485 certification. | 1 G | 18700 | 请咨询 |
| 3208796 | Sigma-Aldrich | Reagents designed and manufactured under current ISO 13485 certification. | 100 MG | 2520 | 请咨询 |
| 3208796 | Sigma-Aldrich | Reagents designed and manufactured under current ISO 13485 certification. | 500 MG | 10100 | 请咨询 |
| 3447385 | Ryon | 层析纯,30u/mg | 100毫克 | 172 | 请咨询 |
| 3447385 | Ryon | 层析纯,30u/mg | 1克 | 1320 | 请咨询 |
| 3447385 | Ryon | 层析纯,30u/mg | 500毫克 | 747 | 请咨询 |
| 3487648 | Ryon | 试剂级,600u/ml | 1毫升 | 114 | 请咨询 |
| 3487648 | Ryon | 试剂级,600u/ml | 2毫升 | 206 | 请咨询 |
| 3487648 | Ryon | 试剂级,600u/ml | 5毫升 | 420 | 请咨询 |
| 3522557 | J&K | Reagents designed and manufactured under current ISO 13485 certification. | 250MG | 1040 | 请咨询 |
| 3522557 | J&K | Reagents designed and manufactured under current ISO 13485 certification. | 500MG | 1560 | 请咨询 |
| 3522557 | J&K | Reagents designed and manufactured under current ISO 13485 certification. | 50MG | 285 | 请咨询 |
| 3540718 | SERVA | Reagents designed and manufactured under current ISO 13485 certification. | 25MG | 690 | 请咨询 |
| 3540719 | SERVA | Reagents designed and manufactured under current ISO 13485 certification. | 100MG | 1510 | 请咨询 |
| 3540720 | SERVA | Reagents designed and manufactured under current ISO 13485 certification. | 500MG | 4420 | 请咨询 |
| 3555558 | SERVA | Reagents designed and manufactured under current ISO 13485 certification. | 100MG | 1380 | 请咨询 |
| 3555559 | SERVA | Reagents designed and manufactured under current ISO 13485 certification. | 500MG | 4100 | 请咨询 |
| 4010795 | J&K | Reagents designed and manufactured under current ISO 13485 certification. | 100MG | 448 | 请咨询 |
| 4082462 | Millipore | Reagents designed and manufactured under current ISO 13485 certification. | 500 MG | 6280 | 请咨询 |
| 4103171 | Sigma-Aldrich | Reagents designed and manufactured under current ISO 13485 certification. | 100 MG | 2350 | 请咨询 |
| 4171438 | Amethyst | Reagents designed and manufactured under current ISO 13485 certification. | 100MG | 268 | 请咨询 |
| 4171438 | Amethyst | Reagents designed and manufactured under current ISO 13485 certification. | 1G | 1920 | 请咨询 |
| 4171438 | Amethyst | Reagents designed and manufactured under current ISO 13485 certification. | 25MG | 78 | 请咨询 |
| 4171438 | Amethyst | Reagents designed and manufactured under current ISO 13485 certification. | 500MG | 1110 | 请咨询 |
基本信息
| Enzyme Commission (EC) Number【酶学委员会(EC)编号】 | 3.4.21.64 ( BRENDA | IUBMB ) |
| EC number【EC 号】 | 254-457-8 |
| MDL number | MFCD00132129 |
| NACRES | NA.32 |
| Application【应用】 | 在分离DNA和RNA的过程中，用于核酸酶的蛋白水解失活。 用于去除内毒素结合的阳离子蛋白，如溶菌酶和核糖核酸酶A。 据报道，其可用于分离肝、酵母和绿豆线粒体 用于测定酶在细胞膜上的定位 用于石蜡包埋组织切片的处理，暴露用于抗体标记的抗原结合位点。 用于可传播海绵状脑病(TSE)研究中朊病毒脑组织样本蛋白的消化。 |
| Biochem/physiol Actions【生化/生理作用】 | 蛋白酶K是一种稳定且具有高活性的丝氨酸蛋白酶。晶体和分子结构研究证据表明该酶属于枯草素家族，具有活性位点催化三联体(Asp39-His69-Ser224)。它在一系列广泛的环境中均稳定：pH，缓冲盐，去污剂(SDS)以及温度。 在0.1-0.5% SDS存在的情况下，蛋白酶K可保持活性，在不损害分离DNA完整性的前提下，可以消化DNA制剂中的多种蛋白质和核酸酶。 |
| Unit Definition【单位定义】 | 在pH 7.5，37 ℃ 条件下，一单位的酶每分钟将会尿素变性的血红蛋白以产生与1.0 μ摩尔 (181 μg) 酪氨酸等同的颜色(Folin-Ciocalteu试剂提供的颜色)。 |
| Legal Information【法律信息】 | PicoGreen is a registered trademark of Life Technologies |
产品性质
| Quality Level【质量水平】 | 500 |
| grade【等级】 | for molecular biology |
| form【形式】 | buffered aqueous glycerol solution |
| mol wt【分子量】 | 28.93 kDa |
| concentration【浓度】 | ≥10 mg/mL protein ≥800 unit/mL |
| impurities【杂质】 | ≤500 pg/mg DNA (PicoGreen® assay) |
| application(s) | diagnostic assay manufacturing |
| foreign activity【异质活性】 | DNAse, RNAse, exonuclease, endonuclease, and nickase, none detected |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | 2-8℃ |
安全信息
| Pictograms【象形图】 | GHS07,GHS08 |
| Signal word【警示用语：】 | Danger |
| Hazard Statements | H315 - H319 - H334 - H335 |
| Precautionary Statements | P302 + P352 - P305 + P351 + P338 |
| Hazard Classifications【危险分类】 | Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3 |
| Target Organs【靶器官】 | Respiratory system |
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 1 |