谷氨酸-草酰乙酸转氨酶 来源于猪心脏

2.6.1.1 ( BRENDA | IUBMB )

232-571-9

MFCD00131191

NA.54

Glutamic-Oxalacetic Transaminase is found in all tissues and is predominant in the liver and skeletal muscle. It is a prototype of fold-type I pyridoxal 5′-phosphate (PLP)-enzymes. Glutamic-oxalacetic transaminase is a homodimer containing large and small domains in each subunit.

Glutamic-Oxalacetic Transaminase from porcine heart has been used:

• in an enzyme-coupled assay for evaluating L-asparaginase enzyme activity
• in microtiter plate format enzyme-based assay to estimate malic acid content in berry samples
• as a supplement in homogenization buffers for the isolation of heart mitochondrial membranes in the presence of oxaloacetate (OAA)-depleting system

Glutamic-oxalacetic transaminase or aspartate aminotransferase (AAT) catalyzes the interconversion of L-aspartate and α-ketoglutarate with oxalacetate and L-glutamate. This reversible transaminase reaction is dependent on pyridoxal 5′-phosphate (PLP). Glutamic-oxalacetic transaminase maintains the nitrogen currency for metabolism by generating L-glutamate. Elevated serum levels of glutamic-oxalacetic transaminase are indicated in myocardial infarction, liver diseases, and some renal diseases.

一个酶活性单位是指在pH 7.5、37 ℃下，在 L-天冬氨酸存在时，每分钟将1.0 μmole的α-酮戊二酸转化为L-谷氨酸所需的酶量。在25 ℃下，一个单位相当于约2,000 O.D.(Karmen)单位。

含 0.05M 马来酸盐和 2.5mM α-酮戊二酸盐的 3.0M (NH₄)₂SO₄ 中的悬浮液，pH 6.0

由缩二脲确定的蛋白质。

200

Type I

ammonium sulfate suspension

200-500 units/mg protein

glutamic-pyruvic transaminase ≤0.03%
lactic dehydrogenase ≤0.01%
malic dehydrogenase ≤0.01%

wet ice

2-8℃

10000 units in serum bottle

13 - Non Combustible Solids

WGK 3

Eyeshields, Gloves, type N95 (US)