Glyoxalase I from Saccharomyces cerevisiae
产品价格
| 产品编号 | 品牌 | 包装单位 | 单价(元) | 现货 |
| 3091790 | Sigma-Aldrich | 200 UNITS | 1920 | 请咨询 |
| 3091790 | Sigma-Aldrich | 500 UNITS | 3320 | 请咨询 |
| 3091790 | Sigma-Aldrich | 1000 UNITS | 6670 | 请咨询 |
| 3091790 | Sigma-Aldrich | 2500 UNITS | 12370 | 请咨询 |
基本信息
| Enzyme Commission (EC) Number【酶学委员会(EC)编号】 | 4.4.1.5 ( BRENDA | IUBMB ) |
| MDL number | MFCD00131225 |
| NACRES | NA.54 |
| General description【一般描述】 | Glyoxalase detoxification system consists of glyoxalase (GLO)-I and GLO-II. GLO-I is a cytosolic, 42 kDa, dimeric Zn2+ metalloenzyme. |
| Application【应用】 | Glyoxalase I from Saccharomyces cerevisiae has been used as a standard in Glyoxalase I assay for C. dilatata females and brooded encapsulated embryos. It has also been used as a standard for calibration curve generation for quantifying glyoxalase I from brain tissues and cerebral microvessels. |
| Biochem/physiol Actions【生化/生理作用】 | Glyoxalase I is universally expressed and involved in the protection against cellular damage due to cytotoxic metabolites such as advanced glycation end products (AGEs). It is an integral component of the detoxification system, catalyzing the conversion of reactive, acyclic a-oxoaldehydes into the corresponding a-hydroxyacids in a glutathione-dependent manner. |
| Unit Definition【单位定义】 | One unit will form 1.0 μmole of S-lactoylglutathione from methylglyoxal and reduced glutathione per min at pH 6.6 at 25 ℃. |
| Physical form【外形】 | Solution in 50% glycerol, 0.4 M (NH4)2SO4 and 0.002 M KH2PO4 pH 6.5 |
| Analysis Note【分析说明】 | Protein determined by biuret. |
产品性质
| Quality Level【质量水平】 | 200 |
| type【类型】 | Grade IV |
| form【形式】 | buffered aqueous glycerol solution |
| Specific Activity | ≥400 units/mg protein |
| foreign activity【异质活性】 | glyoxalase II ≤1% |
| storage temp.【储存温度】 | 2-8℃ |
| packaging【包装】 | 500, 1000, 2500 units in glass bottle |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |