产品介绍 荷兰Lumicks m-Trap超高分辨率双光镊
技术特征
技术原理 Lumicks超高分辨率双光镊主要由微液流控制系统、光镊操纵系统、力学检测系统组成。微液流控制系统采用分通道集成设计,避免反应体系交叉污染,确保多步骤生物反应原位进行;光镊系统通过高度聚焦激光束产生的力来操作纳米或微米级的介电质颗粒,实现了对生物分子的单分子水平的操纵。 应用领域 应用案例 蛋白折叠: 10秒钟之内单个钙调蛋白分子结构的变化追踪。灰色数据表示2.5kHz采样率,红色为200Hz。柱形图显示钙调蛋白分子主要存在两种活性状态,而第三种瞬时性的状态可以忽略。 规格参数
文献列表 1. Zhang S, Zhang Q, Hou X M, et al. Dynamics of Staphylococcus aureus Cas9 in DNA target Association and Dissociation. EMBO reports, 2020 2. Zhang Q, Wen F, Zhang S, et al. The post-PAM interaction of RNA-guided spCas9 with DNA dictates its target binding and dissociation. Science advances, 2019 3. Brouwer I, Sitters G, Candelli A, et al. Sliding sleeves of XRCC4–XLF bridge DNA and connect fragments of broken DNA. Nature, 2016 4. Kurniawan N A, Vos B E, Biebricher A, et al. Fibrin networks support recurring mechanical loads by adapting their structure across multiple scales. Biophysical journal, 2016 5. Candelli A, Holthausen J T, Depken M, et al. Visualization and quantification of nascent RAD51 filament formation at single-monomer resolution. Proceedings of the National Academy of Sciences, 2014 6. Heller I, Sitters G, Broekmans O D, et al. STED nanoscopy combined with optical tweezers reveals protein dynamics on densely covered DNA. Nature methods, 2013. 各大用户遍布全球 |
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