Creatinine Deiminase microbial

3.5.4.21 ( BRENDA | IUBMB )

253-449-1

MFCD00130878

NA.54

Creatinine Deiminase microbial has been used:

• to immobilize aminosilylated glass beads based biosensor for ammonia/ammonium and creatinine detection in urine
• in creating creatinine-sensing membrane for biophysical studies
• to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples

Creatinine deiminase has been used in a study to assess the application of a creatinine-sensitive biosensor for hemodialysis control. Creatinine deiminase has also been used in a study to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples.

Creatinine deiminase catalyzes the hydrolysis of creatinine to methylhydantoine and ammonia.

Isoelectric point : 4.4
Michaelis constant : 3.5 x 10‾³M (Creatinine)
Structure : 6 subunits per mol of enzyme
Inhibitors : Ag⁺,Hg⁺⁺, o-phenanthroline,monoiodoacetate
Optimum pH : 8.5 – 9.5
Optimum temperature : 65 – 75℃
pH Stability : pH 7.0 – 11.0 (30℃, 20hr)
Thermal stability : Below 65℃ (pH 7.5, 1hr)

One unit will hydrolyze 1.0 μmole of creatinine to N-methylhydantoin and NH₃ per min at pH 7.5 at 37 ℃ in a coupled system with L-glutamic dehydrogenase.

Lyophilized powder containing mannitol as stabilizer

lyophilized powder

200

≥25 units/mg protein

~260 kDa

Protein, ≥15% biuret

−20℃

250 units in poly bottle

13 - Non Combustible Solids

WGK 3

Eyeshields, Gloves, type N95 (US)