肌酸脱水酶 来源于放线杆菌 属
Creatinase from microorganisms
产品价格
| 产品编号 | 品牌 | 纯度规格 | 包装单位 | 单价(元) | 现货 |
| 3072711 | Sigma-Aldrich | lyophilized powder, ≥4 units/mg solid | 1000 UNITS | 5040 | 请咨询 |
| 3072712 | Sigma-Aldrich | lyophilized powder, ≥4 units/mg solid | 500 UNITS | 3720 | 请咨询 |
| 3447457 | Ryon | BR，5u/mg | 500U | 300 | 请咨询 |
| 3447457 | Ryon | BR，5u/mg | 1KU | 480 | 请咨询 |
| 3447457 | Ryon | BR，5u/mg | 5KU | 1650 | 请咨询 |
基本信息
| Enzyme Commission (EC) Number【酶学委员会(EC)编号】 | 3.5.3.3 ( BRENDA | IUBMB ) |
| EC number【EC 号】 | 253-471-1 |
| MDL number | MFCD00130875 |
| NACRES | NA.54 |
| General description【一般描述】 | Creatinase is a homodimer that catalyzes hydrolysis of creatine. It consists of two monomer subunit and two defined domains; N and C terminal domains. C terminal fold have both the α helices and anti-parallel β sheet within two structurally similar domains. In between these two domains, there is a sulfhydryl group that acts as active site and the activity is metal independent. |
| Application【应用】 | Creatinase mixed with sarcosine oxidase may be used to determine the level of creatine in different pH, temperature, enzyme ratio, and buffer concentration. It may also be used to determine the plasma creatinine level by using a centrifugal analyser. |
| Biochem/physiol Actions【生化/生理作用】 | Creatinase accelerates the conversion reaction of creatine and water molecule to sarcosine and urea. It always acts in homodimer state and is induced by choline chloride. |
| Physical properties【物理属性】 | Isoelectric point: 4.6 ± 0.1 Michaelis constant: 1.9 x 10‾2M (Creatine) Structure: 2 subunits per mole of enzyme Inhibitors: Cu++, Hg++, Ag+ Optimum pH: 8.0 Optimum temp: 40℃ pH Stability: pH 5.5 − 9.0 (25℃, 16hr) Thermal stability: Below 50℃ (pH 7.5, 30 min) |
| Unit Definition【单位定义】 | One unit will hydrolyze 1.0 μmole of creatine to urea and sarcosine per min at pH 7.5 at 37 ℃. |
| Physical form【外形】 | Lyophilized powder containing sugars and EDTA as stabilizers |
产品性质
| Quality Level【质量水平】 | 200 |
| biological source【生物来源】 | bacterial (Actinobacillus spp.) |
| form【形式】 | lyophilized powder |
| Specific Activity | ≥4 units/mg solid |
| mol wt【分子量】 | ~100 kDa |
| storage temp.【储存温度】 | −20℃ |
| packaging【包装】 | 1000 units in poly bottle |
安全信息
| Storage Class Code【储存分类代码】 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, type N95 (US) |