甘露醇脱氢酶 来源于肠系膜明串珠菌

Mannitol Dehydrogenase from Leuconostoc mesenteroides
CAS NO:9001-65-4
规格:lyophilized powder, ≥60 units/mg protein
包装规格:5 UNITS,50 UNITS
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产品价格
产品编号品牌包装单位单价(元)现货
3107912Sigma-Aldrich5 UNITS3430请咨询
3107912Sigma-Aldrich50 UNITS17050请咨询
基本信息
Enzyme Commission (EC) Number【酶学委员会(EC)编号】
1.1.1.67 ( BRENDA | IUBMB )
MDL number
MFCD00131575
NACRES
NA.54
General description【一般描述】
Mannitol dehydrogenase (MDH) is a NAD-dependent enzyme, obtained from several organisms like, Lactobacillus brevis, Leuconostoc mesenteroides, Absidia glauca and Rhodococcus. MDH obtained from L. mesenteroides exhibits similarity in sequence with the medium-chain dehydrogenase/reductase protein family. It also contains four identical subunits of about 38 kDa in size.
The molecular weight of mannitol dehydrogenase is 136 kDa.
Mannitol dehydrogenase is a cytoplasmic enzyme whose primary role is the regulation of intracellular levels of the sugar alcohol mannitol in plants. It catalyzes the pyridine nucleotide dependent reduction of fructose to mannitol.
Application【应用】
It has been used in a study to assess glucosylglycerol and glucosylglycerate as enzyme stabilizers.
Can be used for the production of D-mannitol.
This preparation is useful in the determination of mannitol in urine.
Biochem/physiol Actions【生化/生理作用】
Mannitol dehydrogenase (MDH) helps to catalyze the pyridine nucleotide-dependent oxidation of D-mannitol to D-fructose. Mannitol dehydrogenase (MDH) obtained from L. mesenteroides is highly specific for the conversion fructose and mannitol.
Unit Definition【单位定义】
在 pH 5.3 和 30℃ 且存在 NADH 的条件下,一单位本品每分钟将还原 1.0μmol D-果糖。如上定义的一单位酶相当于如 Lunn 等人所述的约 1.8 单位酶,并且在 pH 8.6 和 40℃ 且存在 NAD 的条件下,一单位酶每分钟将氧化 1.0μmol D-甘露醇。
Physical form【外形】
含缓冲盐、磷酸钾以及二硫苏糖醇的冻干粉
Preparation Note【制备说明】
部分纯化
Analysis Note【分析说明】
根据 Yamanaka, Meth. Enzymol., 41(B), 138 (1975) 所述方法进行分析。
产品性质
form【形式】
lyophilized powder
Quality Level【质量水平】
200
Specific Activity
≥60 units/mg protein
mol wt【分子量】
136 kDa
storage temp.【储存温度】
−20℃
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 3
Personal Protective Equipment【个人防护装备】
Eyeshields, Gloves
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