神经氨酸酶 来源于产气荚膜梭菌(韦氏梭菌)
Neuraminidase from Clostridium perfringens (C. welchii)
产品价格
| 产品编号 | 品牌 | 纯度规格 | 包装单位 | 单价(元) | 现货 |
| 3173006 | Sigma-Aldrich | Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder | 625 UNITS | 20770 | 请咨询 |
| 3173006 | Sigma-Aldrich | Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder | 2.5 UNITS | 817 | 请咨询 |
| 3173006 | Sigma-Aldrich | Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder | 25 UNITS | 2160 | 请咨询 |
| 3173006 | Sigma-Aldrich | Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder | 250 UNITS | 14380 | 请咨询 |
| 3173006 | Sigma-Aldrich | Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder | 6 UNITS | 950 | 请咨询 |
| 3173007 | Sigma-Aldrich | Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder | 10 UNITS | 6910 | 请咨询 |
| 3173007 | Sigma-Aldrich | Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder | 4 UNITS | 3650 | 请咨询 |
| 3173008 | Sigma-Aldrich | Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder | 1 UNIT | 2160 | 请咨询 |
| 3173008 | Sigma-Aldrich | Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder | 10 UNITS | 11450 | 请咨询 |
| 3173008 | Sigma-Aldrich | Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder | 5 UNITS | 6210 | 请咨询 |
| 3173008 | Sigma-Aldrich | Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder | 50 UNITS | 39450 | 请咨询 |
| 3173009 | Sigma-Aldrich | Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder | 1 UNIT | 2790 | 请咨询 |
| 3173009 | Sigma-Aldrich | Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder | 10 UNITS | 15430 | 请咨询 |
| 3173009 | Sigma-Aldrich | Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder | 5 UNITS | 9140 | 请咨询 |
| 3173009 | Sigma-Aldrich | Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder | 50 UNITS | 52490 | 请咨询 |
| 3173010 | Sigma-Aldrich | Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder | 1 UNIT | 2270 | 请咨询 |
| 3173010 | Sigma-Aldrich | Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder | 2 UNITS | 3820 | 请咨询 |
| 3173011 | Sigma-Aldrich | Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder | 1 UNIT | 2290 | 请咨询 |
| 3173012 | Sigma-Aldrich | Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder | 1 ML | 3280 | 请咨询 |
| 3335820 | Sigma-Aldrich | Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder | .2 UNIT | 12430 | 请咨询 |
| 3335822 | Sigma-Aldrich | Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder | 1 SET | 4510 | 请咨询 |
| 3335823 | Sigma-Aldrich | Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder | 0.2 UNIT | 7100 | 请咨询 |
| 3447239 | Ryon | BR，300u/mg | 100U | 240 | 请咨询 |
| 3447239 | Ryon | 梭菌来源，10u/mg | 10U | 690 | 请咨询 |
| 3447239 | Ryon | BR，300u/mg | 1KU | 1320 | 请咨询 |
| 3447239 | Ryon | BR，300u/mg | 250U | 480 | 请咨询 |
| 3447239 | Ryon | 梭菌来源，10u/mg | 25U | 1650 | 请咨询 |
| 4143699 | Sigma-Aldrich | Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder | 500 MIU | 5920 | 请咨询 |
基本信息
| Enzyme Commission (EC) Number【酶学委员会(EC)编号】 | 3.2.1.18 ( BRENDA | IUBMB ) |
| EC number【EC 号】 | 232-624-6 |
| MDL number | MFCD00131711 |
| NACRES | NA.54 |
| General description【一般描述】 | 神经氨酸酶是一种水解酶，其可促进流感病毒从感染细胞释放并促进病毒的传播。 |
| Application【应用】 | 来自产气荚膜梭菌的神经氨酸酶已经用于评估神经氨酸酶预处理的绵羊中人T淋巴细胞的结合。 它也被用于研究胆汁盐对神经氨酸酶水解作用的影响。 |
| Biochem/physiol Actions【生化/生理作用】 | 神经氨酸酶可以通过去除细胞表面上暴露的带负电荷的唾液酸残基来增加某些细胞系中的聚集。 使用神经氨酸酶去除细胞表面上糖蛋白的唾液酸残基已有多次报道。通常，实验步骤中会表明将神经氨酸酶在PBS中，37℃条件下孵育30分钟进行使用，然后用PBS洗涤几次。用浓度低得多的神经氨酸酶处理组织切片需要更长的温育时间：在pH 4.2-5的0.1M乙酸盐缓冲液中，对于1-4U/mL浓度，需要在37℃下孵育2-20小时。 利用神经氨酸酶裂解唾液酸基团已用来研究糖蛋白结构的抗体识别。与其他两种方法进行比较，使用神经氨酸酶可更好的对N-乙酰神经氨酸进行估测。 神经氨酸酶可用于从多种糖蛋白中切割末端N-乙酰神经氨酸(唾液酸)。 来自产气荚膜梭菌的酶可切割末端唾液酸残基，这些残基可通过α-2,3-、α-2,6-或α-2,8-与Gal、GlcNac、GalNAc、AcNeu、GlcNeu、寡糖、糖脂或糖蛋白进行连接。切割的相对速率按以下顺序逐渐降低：α-2-3>α-2-6。α-2-8。与来自A. ureafaciens的神经氨酸酶(Sigma N3642)(优先切割α-2-6连接的残基)相比，来自产气荚膜梭菌的神经氨酸酶可最有效地切割α-2-3连接的唾液酸残基。 |
| Unit Definition【单位定义】 | 在37℃、pH 5.0的条件下，一个单位每分钟可从牛下颌粘蛋白中释放1.0微摩尔的N-乙酰神经氨酸。以4MU-NANA为底物， 一个单位在37℃、pH 5.0的条件下，每分钟可释放1.0微摩尔的2′-(4-甲基伞形酮)-a-D-N-乙酰基神经氨酸。 |
| Preparation Note【制备说明】 | 其通过盐分馏进行制备。 |
| Analysis Note【分析说明】 | 按照4MU-NANA单位进行包装 |
产品性质
| Quality Level【质量水平】 | 400 |
| type【类型】 | Type V |
| form【形式】 | lyophilized powder |
| Specific Activity | ≥0.1 units/mg solid (using mucin) ≥1.3 units/mg solid (using 4MU-NANA) |
| application(s) | diagnostic assay manufacturing |
| foreign activity【异质活性】 | Protease and NAN-aldolase, present |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | Clostridium perfringens str. 13 ... nanI(988807) |
| packaging【包装】 | 2.5, 6 units in glass bottle 25, 250 units in poly bottle |
安全信息
| Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
| WGK | WGK 3 |
| Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, type N95 (US) |