N-Acetylneuraminic Acid Aldolase from microorganisms

CAS NO:9027-60-5
规格:,BR,50u/mg
包装规格:25 UNITS,1KU
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产品价格
产品编号品牌纯度规格包装单位单价(元)现货
3168744Sigma-Aldrichlyophilized powder, ≥20 units/mg protein (biuret)25 UNITS11480请咨询
3486168RyonBR,50u/mg1KU1100请咨询
基本信息
Enzyme Commission (EC) Number【酶学委员会(EC)编号】
4.1.3.3 ( BRENDA | IUBMB )
Beilstein
2697172
MDL number
MFCD00131656
NACRES
NA.32
Application【应用】
This enzyme is useful for enzymatic determination of N-acetylneuraminic acid and sialic acid when
coupled with the related enzymes in clinical analysis.
For industrial use, this enzyme is useful for enzymatic synthesis of sialic acid.
Used in the Sialic Acid Quantification Kit, SIALIC-Q
Physical properties【物理属性】
Isoelectric point: 4.6 ± 0.1
Michaelis constant: 2.5 x 10‾3M (N-Acetylneuraminic acid)
Structure: 3 subunits (approx. 35,000) per mol of enzyme
Inhibitors: p-Chloromercuribenzoate, sodium dodecyl sulfact, Hg++, Ag+
Optimum pH: 7.5– 8.0
Optimum temp: 70℃
pH Stability: pH 6.0–9.0 (10℃, 25hr)
Thermal stability: Below 65℃ (pH 7.5, 30 min)
Unit Definition【单位定义】
One unit will release 1.0 μmole of pyruvate from NANA per min at pH 7.7 at 37 ℃.
Physical form【外形】
Lyophilized powder containing mannitol and EDTA
产品性质
form【形式】
lyophilized powder
Quality Level【质量水平】
200
Specific Activity
≥20 units/mg protein (biuret)
mol wt【分子量】
~98 kDa
storage temp.【储存温度】
−20℃
packaging【包装】
25 units in glass bottle
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 3
Personal Protective Equipment【个人防护装备】
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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