Dextran Sucrase from Leuconostoc mesenteroides
产品价格
| 产品编号 | 品牌 | 包装单位 | 单价(元) | 现货 |
| 3075258 | Sigma-Aldrich | 10 UNITS | 5110 | 请咨询 |
基本信息
| Enzyme Commission (EC) Number【酶学委员会(EC)编号】 | 2.4.1.5 ( BRENDA | IUBMB ) |
| MDL number | MFCD00130936 |
| NACRES | NA.54 |
| General description【一般描述】 | Dextran Sucrase from Leuconostoc mesenteroides belongs to glycoside hydrolase family 70 (GH70). It functions through a retaining mechanism and uses two catalytic acidic residues. Dextran sucrase has a dextran binding site in the C-terminal domain. |
| Application【应用】 | Dextran sucrase from Leuconostoc mesenteroides has been used in a study to investigate the functional and structural characterization of α-(1→2) branching sucrase derived from DSR-E glucansucrase. Dextran sucrase from Leuconostoc mesenteroides has also been used in a study to investigate the bioengineering of Leuconostoc mesenteroides glucansucrases. The enzyme from Sigma has been used to prepare immobilized sphere for the production of dextran from sucrose. Dextran Sucrase from Leuconostoc mesenteroides has been used:
|
| Biochem/physiol Actions【生化/生理作用】 | Dextransucrases are glucansucrases that are able to produce dextran, a glucose polymer linked mainly through α1-6 bonds. However, α1-3, α1-6, α1-4 and α1-2 bonds are also found, in both the main chain and the branching linkages. The peptide has approximately 1600 amino acids. The aspartic acid in position 551 is essential for catalytic activity, while glutamic acid 589 and aspartic acid 662 complement the catalytic triad. The activity of dextransucrase is decreased by EDTA, and is restored by the addition of calcium ions. Zinc, cadmium, lead, mercury and copper ions are inhibitory to various degrees. |
| Quality【质量】 | 色谱纯化产品 |
| Unit Definition【单位定义】 | One unit will liberate 1.0 μmole of fructose per min at 37 ℃, pH 5.4. |
| Physical form【外形】 | Lyophilized powder containing dextran, MES buffer salts and CaCl2 |
产品性质
| form【形式】 | lyophilized powder |
| Quality Level【质量水平】 | 200 |
| Specific Activity | ≥100 units/mg protein |
| composition【组成】 | Protein, ~15% Lowry |
| solubility【溶解性】 | H2O: soluble 0.9-1.1 mg/mL, clear to slightly hazy, colorless to light yellow |
| storage temp.【储存温度】 | −20℃ |
| packaging【包装】 | 10 units in glass bottle |
安全信息
| Storage Class Code【储存分类代码】 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, type N95 (US) |